dynactin complex components Search Results


prota-3s  (BioTools Inc)
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BioTools Inc prota-3s
Prota 3s, supplied by BioTools Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nb110-55563  (Bio-Techne corporation)
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Bio-Techne corporation nb110-55563
Nb110 55563, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynactin complex components  (Addgene inc)
93
Addgene inc dynactin complex components
A) TZM-BL cells expressing or not Trim69 were challenged with GFP-coding and JR-FL Env-bearing single round of infection-competent HIV-1 virus following siRNA transfection of the indicated proteins (efficacy of silencing presented in Extended Data 9B). The extent of infection was measured by flow cytometry two to three days later. B) Schematic organization of the dynein and <t>dynactin</t> complexes along with the BICD2 adaptor. Red arrows indicate the <t>components</t> analyzed by confocal microscopy using either antibodies directed against endogenous proteins ( C) BICD2 and Extended Data 10 for DYNLL1), or using ectopic transfection of GFP- or mCherry-fusion components ( D ). The complete panel analysis is provided in the Extended Data 10). E) TZM-BL cells expressing or not DYNC1LI2 along with the SPRY-7A Trim69 mutant were obtained by transient transfection. Cells were then analyzed by confocal microscopy. The graph present averages and SEM while panels present typical images obtained (n= 3). * and ns, p<0.05 and non-significant following one-way Anova, Tukey’s multiple comparison tests between the indicated conditions.
Dynactin Complex Components, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
dynactin complex components - by Bioz Stars, 2026-04
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A) TZM-BL cells expressing or not Trim69 were challenged with GFP-coding and JR-FL Env-bearing single round of infection-competent HIV-1 virus following siRNA transfection of the indicated proteins (efficacy of silencing presented in Extended Data 9B). The extent of infection was measured by flow cytometry two to three days later. B) Schematic organization of the dynein and dynactin complexes along with the BICD2 adaptor. Red arrows indicate the components analyzed by confocal microscopy using either antibodies directed against endogenous proteins ( C) BICD2 and Extended Data 10 for DYNLL1), or using ectopic transfection of GFP- or mCherry-fusion components ( D ). The complete panel analysis is provided in the Extended Data 10). E) TZM-BL cells expressing or not DYNC1LI2 along with the SPRY-7A Trim69 mutant were obtained by transient transfection. Cells were then analyzed by confocal microscopy. The graph present averages and SEM while panels present typical images obtained (n= 3). * and ns, p<0.05 and non-significant following one-way Anova, Tukey’s multiple comparison tests between the indicated conditions.

Journal: bioRxiv

Article Title: Microtubule remodeling by the innate immune factor Trim69 compromises dynein-dependent migration of HIV virion cores towards the nucleus

doi: 10.1101/2025.02.06.636796

Figure Lengend Snippet: A) TZM-BL cells expressing or not Trim69 were challenged with GFP-coding and JR-FL Env-bearing single round of infection-competent HIV-1 virus following siRNA transfection of the indicated proteins (efficacy of silencing presented in Extended Data 9B). The extent of infection was measured by flow cytometry two to three days later. B) Schematic organization of the dynein and dynactin complexes along with the BICD2 adaptor. Red arrows indicate the components analyzed by confocal microscopy using either antibodies directed against endogenous proteins ( C) BICD2 and Extended Data 10 for DYNLL1), or using ectopic transfection of GFP- or mCherry-fusion components ( D ). The complete panel analysis is provided in the Extended Data 10). E) TZM-BL cells expressing or not DYNC1LI2 along with the SPRY-7A Trim69 mutant were obtained by transient transfection. Cells were then analyzed by confocal microscopy. The graph present averages and SEM while panels present typical images obtained (n= 3). * and ns, p<0.05 and non-significant following one-way Anova, Tukey’s multiple comparison tests between the indicated conditions.

Article Snippet: Plasmids expressing the following dynein and dynactin complex components were also obtained through Addgene: DYNC1LI1 and DYNC1LI2 respectively pLVXPuro-mGFP-LIC1/-LIC2 (cat #66600 and #66601).

Techniques: Expressing, Infection, Virus, Transfection, Flow Cytometry, Confocal Microscopy, Mutagenesis, Comparison